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Cyclophosphamide, an alkylating agent that blocks DNA replication, together with Docetaxel, an inhibitor of the dynamics of microtubules, essential components of cell division, is the neoadjuvant chemotherapy regimen with the best pCR in patients with TNBC, with a pCR rate of 50%, compared with very low or zero rates in luminal A and type B subtypes
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For the differentiation assay, neurospheres were plated on poly-lysine/laminin coated coverslips in serum-free, growth-factor-free media as described previously
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