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J Neurol (2022) 269(2):541–76
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2.2.2 Measurement of serum lipocalin, leptin, IL-6, TNF-α, creatinine, urea nitrogen, triglycerides After 12 weeks of intervention, the mice were anesthetized with 1% sodium pentobarbital (100–200 mg/kg body weight), and blood samples were collected through the abdominal aorta, serum was separated, and the serum levels of adiponectin, leptin, IL-6, and TNF-α were measured using ELISA kits, while creatinine, urea nitrogen, and triglycerides were determined using standard automated biochemical assays.2.2.3 24 h protein quantification and urinary albumin