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In vitro kinase assays In total, 300 ng of purified recombinant GST-tagged rat ArfGAP1 proteins (F5 252-359 fragment: WT, S284A, T291A or T292A) were incubated with 95 ng of recombinant GST-tagged human LRRK2 (residues 970–2,527: G2019S or D1994A
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