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LOXL2-DM was used as a negative control in this assay, as the LTQ generation is a self-processing reaction that requires copper binding 52,53 that is lacking in the LOXL2-DM 40
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Briefly, in a 200 μl reaction volume, 5 μg ml −1 recombinant GSTO1 in reaction buffer (100 mM Tris (pH 8.0), 1.5 mM EDTA and 1 mM DTT) was incubated with DMSO or different concentrations of inhibitors for 30 min at 37 °C
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