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blots were incubated with rabbit primary antibody anti-Id2 (c-20) (Santa Cruz Biotechnology, CA, USA), diluted 1∶750 in 1% (w/v) non-fat dry milk TBS-Tween (TTBS) overnight at 4°C, or with α tubulin (Santa Cruz Biotechnology, CA, USA), diluted 1∶1000 in 1% (w/v) non-fat dry milk TTBS overnight at 4°C with shaking

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