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to an OD 600 of 0.7-0.8 ( ⁇ 2-3 h) before induction with 0.5 mM IPTG and incubated for an additional 3 h
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The other prospective study was open-label but differed in two critical ways from the others in its design: first, subcutaneous mB12 was combined with oral folinic acid, and second, only participants with unfavorable biochemical profiles consisting of decreased methylation capacity and decreased GSH redox ratio were entered into the study
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