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( mRNA quantification by quantitative real-time PCR (qRT-PCR) Total RNA was isolated using QIAzol-Reagent (Qiagen, Germany), based on modified Chomczyński & Sacchi’s method (Chomczynski and Sacchi, 2006), following the manufacturer’s protocol

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🔹 Glycine Another key building block When combined with NAC (“GlyNAC”), studies show improved glutathione status and mitochondrial function in older adults (Kumar et al., Clin Transl Med, 2021) 🔹 Selenium Required for glutathione-related enzymes (glutathione peroxidase)2