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Cells were lysed with 500 μL of RIPA lysis buffer containing 150 mM NaCl, 1% NP-40, 0.5% sodium deoxycholate, 0.1% SDS, 25 mM Tris-HCl, supplemented with protease and phosphatase inhibitors at a 1:100 dilution

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Numbness or tingling in hands and feet Peripheral nerve changes are a more serious sign of prolonged deficiency