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RAW264.7 cells were centrifuged at 400 g for 5 min and resuspended in phosphate-buffered saline and were sequentially incubated at 4°C with anti-mouse CD86 (E-AB-F0994E, Elabscience) and then with anti-CD206 (E-AB-F1135D, Elabscience) for 60 min in the dark, according to the manufacturer’s instructions
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