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1.2 mL of 0.5 M phosphate buffer (pH 7.8) and 50 μl of Ellman's reagent were added to the resulting supernatant (0.2 ml)

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Mechanisms Step 5 : intracellular anaerobic bacteria are driving the changes in human host cell metabolism (including glycine, citrate, cholesterol to androstenedione, anaerobic respiration) and methylation impacting through multiple mechanisms on cancer aggression and progression