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Thermal activation was carried out with CBS protein at 1 µM in 25 mM HEPES, pH 7.5, 200 mM NaCl and 50 μM PLP as 50 µl aliquots treated at different temperatures using a VeritiPro thermal cycler (Thermo Fisher Scientific) for 2 min

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Median concentrations of homocysteine, cysteine, choline, and betaine were significantly higher in women with preeclampsia than controls, both in maternal and fetal plasma