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Frequency and duration Typical rodent study dosing frequencies: Daily (most common) — appropriate for 7-30 day healing protocols Twice-daily — used in some acute-phase studies Every other day — occasionally used with higher per-dose amounts Duration by protocol type: Acute injury models (tendon transection, muscle injury): 7-21 days Chronic or progressive models : 21-60 days Long-term safety studies : extended dosing windows up to 90 days in some protocols 6

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