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Cell viability and NO assays BV-2 cells were seeded at a density of 5 × 10 4 cells/mL and exposed to LPS (200 ng/mL), followed by 1 h pretreatment of SE-EE at the indicated concentrations (20, 100 and 200 μg/mL) for 24 h

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One FRAP unit is arbitrarily defined as the reduction of 1 M Fe 3+ to Fe 2+ (MacDonald-Wicks et al