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The unspecific proteins were washed in 10 mM NaP i at pH 8, 150 mM NaCl, 1 mM GSH, 10% glycerol, 50 mM imidazole, and 0.1% reduced Triton X-100 (Sigma) and rat MGST1 protein was eluted in 350 mM imidazole

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Cell 16 2091–2105 10.1091/mbc.E04-08-0737 [DOI] [PMC free article] [PubMed] [Google Scholar] Pompella A., Visvikis A., Paolicchi A., De Tata V., Casini A