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Briefly, the supernatant underwent a series of centrifugation steps at varying speeds and durations, namely 300 g for 10 min, 3000 g for 15 min, and 10,000 g for 60 min at 4 °C, to remove cells and debris, and then filtered using a 0.22 μm filter (Millipore, USA)
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This leads many people to look for ways to replenish their levels through supplementation
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