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Cells were incubated for 2 h at RT with a primary antibody mixture of polyclonal guinea pig anti-Synaptophysin-1 (1:1000, SYSY), polyclonal chicken anti-MAP2 (1:10,000, Abcam), and polyclonal rabbit anti-Munc18-1 antibodies (1:1000, described previously

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A basic timeline is as follows, though results vary based on skin type, pigmentation intensity, and product formulation: 2-3 weeks: a healthy shine and subtle brightness 4-6 weeks: Tan and small spots disappear 8–12 weeks: More uniform tone and noticeable pigmentation lightening Because topical glutathione works with your body's natural melanin cycle rather than bleaching or stripping the skin, results are steady but gradual