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For this, HEK-293T cells were plated (2 × 10 5 cells in a 6-well plate) and grown for 24 h before transfection (using PEI) with pCMV_MTS_NB_4A7, pCMV_MTS_NB_6B1, pCMV_MTS_NB_16C10, and pCMV_MTS_NB_28F6, according to the PEI manufacturer’s instructions (an empty vector was included as a control condition)

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Reduces systemic inflammation One of the most potent peptides for managing chronic inflammation and pain