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Temperature proves most critical - every 10°C increase doubles degradation rate, meaning room temperature storage (25°C) degrades peptides 4-8x faster than refrigeration (2-8°C) and dramatically faster than freezing (-20°C or below)

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(n = 6 for PBS, 8 for α-PD-1, and 7 for Prodrug 201 + α-PD-1 group biologically independent samples)