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The addition of vitamin C conferred only minor alterations in the levels of several key proteins involved in radiation response, proliferation, or apoptosis Western blot assays were performed to analyze the expression of proteins involved in radiation response (γH2AX and p53), proliferation (Cyclin D1), and apoptosis (PARP, and cleaved PARP) in breast cancer cells treated with different combinations of RT and vitamin C as well as RT alone or vitamin C alone (Figure 3)