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HepG2 cells were seeded at 2X10 5 cells per ml well and transfected with 12.5 μg of GeneArt platinum Cas9 nuclease and 144 μL of Cas9 plus reagent or 12.5 μg of GeneArt platinum Cas9 nuclease, 144 μL of Cas9 plus reagent, and 2.5 μg of sgRNA for 9 h as previously described [40]

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