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Plotting all the z-score values for the full screen for the parasite growth parameter (parasites/host nuclei), we found that our positive and negative controls cluster at either ends of the growth spectrum, our non-targeting guides clustered around non-significant z-scores (-2 to 2 exclusive), with our on-targeting guides normally distributed along this entire spectrum (S1J)

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The synthesis of EGT in microbial cells is relatively simple and efficient